Staff Scientist II will utilize their experience and knowledge to work with a cross-functional team of cell and molecular biologists within the Gene Editing Institute. The successful candidate is expected to carry out the assigned projects mostly independently. Primary responsibilities include but are not limited to developing and optimizing CRISPR technology for mammalian cell engineering in vivo and in vitro.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Successful candidates will have a background in molecular biology; including hands-on experience with clone construction, clonal amplification, and tissue culture is desirable. Previous working with AAV, lentiviral vectors, and CRISPR/Cas9 is a plus, but not mandated.
Independently maintain various types of adherent and suspension mammalian cell cultures in a sterile environment
Design and optimize CRISPR components including gRNAs and HDR templates
Perform transfection of mammalian cells utilizing nucleofection, lipofection and other methods
Evaluate the transfections using cell counting and viability assessment
Single cell sorting by FACS and limiting dilutions
Maintenance of laboratory equipment
Interpretation of Sanger sequence data
Maintenance of a highly organized, technical, and detailed laboratory notebook containing protocols used, results derived from each experiment or measurement
Preparation and dissemination of technical reports
Close communication with all involved team members
Present research findings, including preparation of research reports and abstracts, dissemination at national and international scientific conferences and publication of manuscripts by peer-reviewed scientific journals.
Maintain familiarity with current scientific literature and techniques in the field of CRISPR based gene therapy
Perform assigned work safely, adhering to established departmental safety rules and practices; report to Group leader, in a timely manner, any unsafe activities, conditions, hazards, or safety violations that may cause injury to oneself, other employees, patients and visitors.
Performs other related duties as required/assigned.
SCOPE, PURPOSE, AND FREQUENCY OF CONTACTS:
Scientific opinions and discussion to collaborators and customers.
DIRECTION/SUPERVISION OF OTHERS:
Limited supervision of laboratory assistants
Group leader / Director of Gene Editing Institute
EDUCATION AND EXPERIENCE REQUIREMENTS:
Bachelor's degree in biological sciences or related field with 3 - 7 years of work experience or MS with 0 - 4 years of work experience in a biotech setting
Extensive experience in molecular cloning
Extensive experience in mammalian cell culture
Experience in assay development for mammalian cells
Knowledge in primary cell culture
Experience with managing multiple projects simultaneously
Ability to work effectively as part of a team
KNOWLEDGE, SKILL, AND ABILITY REQUIREMENTS:
Knowledge of the expression vectors and the construction
Knowledge in CRISPR genome engineering
Well-versed in culturing adherent and suspension mammalian cells
Skilled in various transfection methodologies
Experienced in in vitro assays using immortalized cancer cells
Experienced in cancer biology/genetics
Proficiency in using Microsoft office and willing to learn new algorithms.
Frequent sitting, standing, and walking with or without reasonable accommodation for physical disabilities
Office, clinical and laboratory settings
Internal Number: JR55520
About ChristianaCare Health System
ChristianaCare Health System is headquartered in Wilmington, Delaware and is one of the country's largest health care providers, ranking 21st in the nation for hospital admissions. Christiana Care is proudly a Nurse Magnet recognized institution. Christiana Care Health System is also one of the largest health care providers in the mid-Atlantic region, serving all of Delaware and portions of seven counties bordering the state in Pennsylvania, Maryland and New Jersey. A not-for-profit, non-sectarian health system, Christiana Care includes two hospitals with more than 1,100 patient beds, and is a major teaching hospital with two campuses. Christiana Care is continually recognized for excellence on a regional and national level. Our role in the community is expressed in the Christiana Care Way: "We serve our neighbors as respectful, expert, caring partners in their health. We do this by creating innovative, effective, affordable systems of care that our neighbors value."Christiana Care is a great place to work because we value diversity and recognize it to be a core part of our success. Because of the diversity of our employees, affiliated health professionals and volunteers, we are ...positioned to meet the unique needs of our patients and community. We acknowledge and celebrate the uniqueness and talent of each employee. Because of our talented workforce we are able to provide a quality healthcare experience to our patients and community. We strive to create an inclusive environment in which individual diversity can be leveraged and thrive. Christiana Care Health System is an equal opportunity employer, firmly committed to prohibiting discrimination, whose staff is reflective of its community and considers qualified applicants for open positions without regard to race, color, sex, religion, national origin, sexual orientation, genetic information, gender identity or expression, age, veteran status, disability, pregnancy, citizenship status, or any other characteristic protected under applicable federal, state, or local law.